Exosome-Depleted UltraGRO™-PURE GI GMP grade

Product Type:Exosome-Depleted, Gamma irradiated FDhPL


Grade:GMP grade

Product Name:Exosome-Depleted UltraGRO™-PURE GI


    • Description:

      Exosome-Depleted UltraGRO™-PURE GI cell culture supplement is a gamma irradiated viral inactivated, exosome-depleted, xeno-free to support cell culture, especially for exosome secretion and isolation assay from research and clinical trials to commercial applications. More than 95% of nanoparticles have been removed from the original human platelet lysate, allowing target cells to produce functional exosomes and other cell-derived extracellular vesicles for further purification, analysis, and therapeutic purposes.

    • Quality Control Test:

      1. Endotoxin: ≤ 10 EU/mL

      2. Sterility: No Growth

      3. Mycoplasma: Negative

      4. Osmolality: 270 - 330 mOsm/Kg

      5. pH: 6.5 - 8.5

      6. Particle depleted rate: >95%

      7. Cell assay: Support MSC culture

      8. Gamma Irradiation Dose: 25~40 kGy

    • Storage and Shipping Information:

      Exosome-Depleted UltraGRO™-PURE GI is most stable when stored frozen until needed. The recommended storage temperature is -20°C. Thaw frozen Exosome-Depleted UltraGRO™-PURE GI product in 37°C water bath before use. Once Exosome-Depleted UltraGRO™-PURE GI is thawed, it is recommended to fully use for completed medium preparation (e.g., 5%) on the same day, or to divide it into single-use aliquots and store unused aliquots at -20°C. The product will be shipped with dry ice.

    • Packaging Information:

      500mL, 100mL, 50mL plastic bottles

    • Media Form:

      Frozen Liquid

    • Download Document:

      AventaCell ED UG-P GI_MSC fact sheet-v2.1_220421


    • Application:

      For human cell culture in producing exosome and other cell-derived extracellular vesicles processing applications.

    • Instructions for Use:

      1. Once the target cells reach desired cell confluency, Exosome-Depleted UltraGRO™-PURE GI supplemented culture media can be introduced to initiate cell-derived EV production.

      2. We recommend applying with 2-5% (v/v) of supplemented media during the culture period. Perform conditioned media harvest and media refresh in every 2-3 days.

    • Cell Lines:

      bone marrow mesenchymal stem cells, adipose tissue derived mesenchymal stem cells, umbilical cord derived mesenchymal stem cells, other mesenchymal stem cells

    • Important Information:

      1. Insoluble particles may form in thawed Exosome-Depleted UltraGRO™- PURE GI. Published research has shown that particles will not alter the performance of the product. It is recommended to remove the precipitates by centrifuge at 3,400 xg for 3~5 minutes. Alternatively, applying 0.22 µm filtration to the prepared Exosome-Depleted UltraGRO™-PURE GI containing medium (e.g., 5%) will not affect the cell culture performance.

      2. 0.22μm filtering is NOT recommended for the 100% Exosome-Depleted UltraGRO™-PURE GI.

      3. Repeated freeze-thaw cycles should be avoided as they will cause an increase in insoluble precipitates.

    • Safety information:

      1. Follow the handling instructions outlined in the Material Safety Date Sheets (MSDS). Wear appropriate protective eyewear, clothing, and gloves.

      2. Exosome-Depleted UltraGRO™-PURE GI is a cell culture supplement collected from healthy donors at FDA licensed centers. Each donor has been tested using FDA licensed tests and found nonreactive for HBsAg, Hepatitis B core antibody (anti-HBc), HIV antibody (anti-HIV-1/2), Hepatitis C antibody (anti-HCV), HTLV-1/2 antibody (anti-HTLV-1/2), Trypanosoma cruzi antibody (anti-T.cruzi), HIV-1, HCV, HBV, WNV nucleic acid testing and Syphilis microhemagglutination test.

      3. This product is manufactured, tested and released in compliance with the relevant GMP guidelines. This product is for in vitro use only.