Products

Exosome-Depleted UltraGRO™-PURE GI Research grade

Product Type:Exosome-Depleted, Gamma irradiated FDhPL

Attribute:Xeno-free

Grade:Research grade

Product Name:Exosome-Depleted UltraGRO™-PURE GI

Catalog Number:HPCHEFRLI50, HPCHEFRLI10, HPCHEFRLI05

    • Description:

      Exosome-Depleted UltraGRO™-PURE GI cell culture supplement is a gamma irradiated viral inactivated, exosome-depleted, xeno-free to support cell culture, especially for exosome secretion and isolation assay from research and clinical trials to commercial applications. More than 95% of nanoparticles have been removed from the original human platelet lysate, allowing target cells to produce functional exosomes and other cell-derived extracellular vesicles for further purification, analysis, and therapeutic purposes.

    • Quality Control Test:

      1. Endotoxin: ≤ 10 EU/mL

      2. Sterility: No Growth

      3. Mycoplasma: Negative

      4. Osmolality: 270 - 330 mOsm/Kg

      5. pH: 6.5 - 8.5

      6. Particle depleted rate: >95%

      7. Cell assay: Support MSC culture

      8. Gamma Irradiation Dose: 25~40 kGy

    • Storage and Shipping Information:

      Exosome-Depleted UltraGRO™-PURE GI is most stable when stored frozen until needed. The recommended storage temperature is -20°C. Thaw frozen Exosome-Depleted UltraGRO™-PURE GI product in 37°C water bath before use. Once Exosome-Depleted UltraGRO™-PURE GI is thawed, it is recommended to fully use for completed medium preparation (e.g., 5%) on the same day, or to divide it into single-use aliquots and store unused aliquots at -20°C. The product will be shipped with dry ice.

    • Packaging Information:

      500mL, 100mL, 50mL plastic bottles

    • Media Form:

      Frozen Liquid

    • Download Document:

      AventaCell ED UG-P GI_MSC fact sheet-v2.1_220421

      [Poster] AventCell 2022 A NEW EXOSOME-DEPLETED XENO-FREE HUMAN PLATELET LYSATE FOR THERAPEUTIC MSC-DERIVED SECRETOME PRODUCTION

    • Application:

      For human cell culture in producing exosome and other cell-derived extracellular vesicles processing applications.

    • Instructions for Use:

      1. Once the target cells reach desired cell confluency, Exosome-Depleted UltraGRO™-PURE GI supplemented culture media can be introduced to initiate cell-derived EV production.

      2. We recommend applying with 2-5% (v/v) of supplemented media during the culture period. Perform conditioned media harvest and media refresh in every 2-3 days.

    • Cell Lines:

      bone marrow mesenchymal stem cells, adipose tissue derived mesenchymal stem cells, umbilical cord derived mesenchymal stem cells, other mesenchymal stem cells

    • Important Information:

      1. Insoluble particles may form in thawed Exosome-Depleted UltraGRO™- PURE GI. Published research has shown that particles will not alter the performance of the product. It is recommended to remove the precipitates by centrifuge at 3,400 xg for 3~5 minutes. Alternatively, applying 0.22 µm filtration to the prepared Exosome-Depleted UltraGRO™-PURE GI containing medium (e.g., 5%) will not affect the cell culture performance.

      2. 0.22μm filtering is NOT recommended for the 100% Exosome-Depleted UltraGRO™-PURE GI.

      3. Repeated freeze-thaw cycles should be avoided as they will cause an increase in insoluble precipitates.

    • Safety information:

      1. Follow the handling instructions outlined in the Material Safety Date Sheets (MSDS). Wear appropriate protective eyewear, clothing, and gloves.

      2. Exosome-Depleted UltraGRO™-PURE GI is a cell culture supplement collected from healthy donors at FDA licensed centers. Each donor has been tested using FDA licensed tests and found nonreactive for HBsAg, Hepatitis B core antibody (anti-HBc), HIV antibody (anti-HIV-1/2), Hepatitis C antibody (anti-HCV), HTLV-1/2 antibody (anti-HTLV-1/2), Trypanosoma cruzi antibody (anti-T.cruzi), HIV-1, HCV, HBV, WNV nucleic acid testing and Syphilis microhemagglutination test.

      3. This product is for in vitro use only.