Products

UltraGRO™ Research grade

Product Type:hPL

Attribute:non-xenogeneic

Grade:Research grade

Product Name:UltraGRO™

Catalog Number:HPCPLCRL50, HPCPLCRL10, HPCPLCRL05

    • Description:

      UltraGRO™ cell culture supplement is a non-xenogeneic, animal serum-free, and heparin-requiring supplement for replacing FBS (fetal bovine serum) to support cell expansion from research use. UltraGRO™ contains abundant growth factors and cytokines necessary for research or industrial cell growth and proliferation of multiple cell types (e.g. MSCs) in research-scale production.

    • Specification:

      1. Appearance: Yellow to Buff liquid

      2. Total Protein: 4.0 - 8.0 g/dL

      3. Endotoxin: ≤ 10 EU/mL

      4. Sterility: Pass

      5. Mycoplasma: Negative

      6. pH: Tested

      7. Osmolality: Tested

      8. Performance Test: Test and record

    • Storage and Shipping Information:

      UltraGRO™ is most stable when stored frozen until needed. The recommended storage temperature is -20°C. Thaw frozen UltraGRO™ product in 37°C water bath before use. Once UltraGRO™ is thawed, it is recommended to fully use for completed medium preparation (e.g. 5%) the same day, or to divide it into single-use aliquots and store unused aliquots at -20°C.

    • Packaging Information:

      500mL, 100mL, 50mL plastic bottles

    • Media Form:

      Frozen Liquid

    • Application:

      For human ex-vivo tissue and cell culture processing applications.

    • Instructions for Use:

      1. UltraGRO™ shows optimal growth of MSC at 5% (v/v) in typical cell culture media, i.e. α-MEM, which contains 2mM L-Glutamine as final concentrate.

      2. We recommend seeding MSCs at approximately 3x103~6x103 per cm2.

      3.  UltraGRO™ requires heparin at a final concentration of 2 IU/mL to be added in the culture media when supplemented with 5% UltraGRO™. Failure to add heparin will result in coagulation during cell culture in typical media.

    • Cell Lines:

      bone marrow mesenchymal stem cells, adipose tissue derived mesenchymal stem cells, umbilical cord derived mesenchymal stem cells, other mesenchymal stem cells.

    • Important Information:

      1. Clotting or insoluble particles may form in thawed UltraGRO™. Published research has shown that particles will not alter the performance of the product.

      2. Clotting or insoluble particles may form in thawed UltraGRO™, it is recommended to centrifuge at 3,400 xg for 3~5 minutes or to filter the liquid concentrate with a sterile 40 μm Cell Strainer to remove insoluble particles.

      3. Filtering the completed medium (e.g. 5%), after UltraGRO™ is diluted in the basal medium, will not affect UltraGRO™ supplemented cell culture performance. However, 0.22 μm filtering is NOT recommended for the 100% UltraGRO™ concentrate, as this may reduce 5% UltraGRO™ cell culture performance.

      4. Repeated freeze-thaw cycles should be avoided as they will cause an increase in insoluble precipitates and resulting potential decrease in UltraGRO™ performance.

    • Safety Packaging information:

      1. Follow the handling instructions outlined in the Material Safety Date Sheets (MSDSs). Wear appropriate protective eyewear, clothing, and gloves.

      2. UltraGRO™ is a cell culture supplement derived from human platelets collected from healthy donors at FDA or HC licensed blood centers. Each donor has been tested using FDA/HC-licensed tests and found nonreactive for HBsAg, Hepatitis B core antibody (anti-HBc), HIV antibody (anti-HIV-1/2), Hepatitis C antibody (anti-HCV), HTLV-1/2 antibody (anti-HTLV-1/2), Trypanosoma cruzi antibody (anti-T. cruzi), HIV-1, HCV, HBV, WNV nucleic acid testing and Syphilis microhemagglutination test.

      3. This product is for in vitro use only.